Objective: The aim of this study was to establish an HPLC method for simultaneous determination of the content of four flavonoid glycosides in Shanlamei Qinggan Cha. Method: Using an Elite hypersil ODS2 column(4.6 mm×250 mm

5 μm)

the mobile phase was acetonitrile-0.1% acetic acid

with flow rate of 1.0 mL·min-1. The detective wavelength was at 344 nm and the column temperature was 35 ℃. Result: The standard curve presented a linear range from 7.012-70.12 mg·L-1 for rutin(r=0.999 6)

the average recovery was 100.68% with RSD of 3.27%.The calibration curve was linear in the range of 5.126-51.26 mg·L-1 for kaempferol-3-O-β-D-galactose -(6-1)- α-L-rhamnoside (r=0.999 9)

the average recovery was 98.48% with RSD of 1.7%. The calibration curve was linear in the range of 2.631-26.31 mg·L-1 for kaempferol-3-O-rutinoside (r=0.999 8)

the average recovery was 101.82% with RSD of 1.9%. The standard curve presented a linear range from 2.634-26.34 mg·L-1 for astragalin (r=0.999 9)

the average recovery was 97.02% with RSD of 1.7%. Conclusion: The method was fast

accurate and simple in good reproducibility

which could be used to control the quality of Shanlamei Qinggan Cha.