Objective: To establish a method for simultaneous determination of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid in Xiongju Shangqing tablets. Method: The separation was performed on a DIKMA Platisil ODS column (4.6 mm×250 mm

5 μm) with gradient elution using methanol and 0.1% H3PO4 aqueous solution as mobile phase. The flow rate was 1 mL·min-1 and the detection wavelengths were 327 nm for chlorogenic acid

berberine and baicalin; 210 nm for oleanolic acid and ursolic acid

respectively. Result: Linear ranges of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid were 0.03-0.6

0.011 4-0.228

0.025-0.5

0.1-2

0.03-0.6 μg

respectively. Their average recoveries were 99.57%(RSD 1.3%)

99.19%(RSD 0.8%)

99.14%(RSD 0.6%)

99.38%(RSD 1.2%)

98.92%(RSD 0.7%)

respectively. Conclusion: The established HPLC method can be used to determine the contents of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid in Xiongju Shangqing tablets. The method can also be used as the standard for the quality control of Xiongju Shangqing tablets.