HPLC测定苏黄止咳胶囊中紫苏醛含量

姚琴; 丰杰; 王想; 赵凤新

doi:10.13422/j.cnki.syfjx.2014240081

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HPLC测定苏黄止咳胶囊中紫苏醛含量

化学与分析 | 更新时间：2024-01-04

- HPLC测定苏黄止咳胶囊中紫苏醛含量
- Determination of Perillaldehyde in Suhuang Zhike Capsule by High-performance Liquid Chromatography
- 中国实验方剂学杂志 2014年20卷第24期页码：81-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项(2010ZX09401-303-2-6)
- DOI：10.13422/j.cnki.syfjx.2014240081
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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姚琴, 丰杰, 王想, 等. HPLC测定苏黄止咳胶囊中紫苏醛含量[J]. 中国实验方剂学杂志, 2014,20(24):81-83.

YAO Qin, FENG Jie, WANG Xiang, et al. Determination of Perillaldehyde in Suhuang Zhike Capsule by High-performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 81-83.
姚琴, 丰杰, 王想, 等. HPLC测定苏黄止咳胶囊中紫苏醛含量[J]. 中国实验方剂学杂志, 2014,20(24):81-83. DOI： 10.13422/j.cnki.syfjx.2014240081.

YAO Qin, FENG Jie, WANG Xiang, et al. Determination of Perillaldehyde in Suhuang Zhike Capsule by High-performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 81-83. DOI： 10.13422/j.cnki.syfjx.2014240081.

摘要

目的: 建立苏黄止咳胶囊中紫苏醛含量的HPLC测定方法. 方法: 采用高效液相色谱法

SHIMADZU Shim-pack VP-ODS C18色谱柱 (4.6 mm × 250 mm

5 μm)

流动相甲醇-水(68:32)

检测波长230 nm

流速0.8 mL·min-1

柱温30 ℃. 结果: 紫苏醛在5.672~453.76 ng与其峰面积呈良好的线性关系

线性回归方程为Y=5.695X+1.28(r=0.999 5)

平均加样回收率为99.57% (RSD 1.7%). 结论: 该方法准确、分离度好、专属性强

可用于苏黄止咳胶囊中紫苏醛的含量测定.

Abstract

Objective: To establish a method for the content determination of perillaldehyde in Suhuang Zhike Capsule. Method: HPLC was performed on SHIMADZU Shim-pack VP-ODS C18 column (4.6 mm × 250 mm

5 μm)

and the mobile phase was consisted of methanol-water (68:32). The detection wavelength was set at 230 nm

the flow rate of 1.0 mL·min-1 and the column temperature was 30 ℃. Result: Perillaldehyde showed a good linear relationship from 5.672 ng to 453.76 ng

and the regression equation was Y=5.695X+1.28 (r=0.999 5). The average recovery was 99.57 % with RSD of 1.68 %. Conclusion: The HPLC method is simply and accurate with a good specificity. It can be used to determine the content of perillaldehyde in the Suhuang Zhike Capsule.

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