Objective: To prepare Gushangning ointments and establish its quality control method. Method: Gushangning ointments was prepared by emulsification method.Qualitative analysis of Gushangning ointments was detected by TLC and precipitation reaction.Contents of notoginsenoside R1 and ginsenoside Rg1

Rb1 in Gushangning ointments by HPLC

mobile phase consisted of acetonitrile(A)-water(B) for gradient elution(0-12 min

19%A;12-60 min

19%-36%A;60-70 min

36%-19%A;70-73 min

19%A)

dection wavelength was set at 203 nm. Result: Gushangning ointments had stable traits

linear ranges of notoginsenoside R1 and ginsenoside Rg1

Rb1 were 0.15-3.75

0.45-11.25

0.42-10.5 μg

average recoveries of these three components were 98.81%(RSD 3.65%)

101.18%(RSD 2.26%)

98.90%(RSD 2.92%)

respectively. Conclusion: This established qualitative analysis is reliable and accurate

HPLC is reliable

simple and precise

which are suitable for quality control of Gushangning ointments.