Objective: To establish a TLC Identification of Trollius chinensis and determination of total flavonoids in the herb. Method: Extraction solvent and deployment system in TLC identification method of T. chinensis was screened by single factor tests.A colorimetry was adopted to determine the content of total flavanoids in T. chinensis by taking orientoside as index component

10% aluminum nitrate solution as color developing reagent and detection wavelength at 510 nm

then content difference of total flavanoids in T. chinensis from different origins was compared. Result: Developing agent was chloroform-methanol-butanone-acetic acid(4:2:3:3)

this established TLC had a good separation effect of each component.Orientoside had a good linear relationship in the range of 8.096-56.672 mg·L-1

average recovery was 98.8%;the mass fractions of total flavanoids in No. 1

2

3

4

5

6 sample were 9.79%

8.71%

8.32%

6.47%

6.42% and 6.71%

respectively. Conclusion: These methods are simple

accurate and applicable for quality control of T. chinensis.