Objective: To analyze components and metabolites in serum of rats after given of Mume Fructus by gavage. Method: Eight female rats were divided into Mume Fructus group and blank control group

former group were given Mume Fructus crude solution(0.633 6 g·mL-1 )2 mL·kg-1 by gavage

the latter with pure water. Four days later

solid phase extraction method was used to extract the drug-containing serum and blank serum

using Mume Fructus extracted by acetonitrile as medicinal materials control. Chromatography using Angient Zorbax Eclipse Plus C8(3.0 mm×100 mm

1.8 μm) for chromatographic column

using 0.1% formic acid solution-acetonitrile gradient elution

flow rate of 0.5 mL·min-1. Mass spectrometry using ESI source

within the scope of 50-1 200 m/z positive scanning mode. Mass spectrum data were preprocessed and principal component analyzed by Mzmine 2.9 software

using the results of principal component analysis and the database of prototype component to identify blood transitional component and metabolites. Result: In serum there were three components from the Mume Fructus original medicinal herbs(such as ursolic acid or oleanolic acid

medicagenic acid)

and there were 10 kinds of the metabolites. Conclusion: This method can be used for analysis of components of medicinal materials into the blood and its metabolites more objective and comprehensive

and it can be used to provide reference and basis for pharmacodynamics research of the Mume Fructus material.