Objective: To study the intestine absorption of punicalagins. Method: The isolated everted intestine model was used to study the absorption of different drug concentration(153.34

301.70 mg·L-1)in different regions of rat intestine(duodenum

jejunum

ileum and colon)

and different time(0

30

60

90

120 min).The sample was determined with HPLC on an YMC-Triart C18 column(4.6 mm×250 mm

5 μm)with methanol-0.1%phosphoric acid. Result: The intestine absorption rate constant increased along with the increase in concentration of punicalagins.Absorption of index components for punicalagins in different intestinal segments had no significant difference

at low concentration

the absorption rate constant is ileum>colon>jejunum>duodenum

at high concentration

jejunum>colon>ileum>duodenum;every intestine segments cunmlative absorption increased along with concentration of punicalagins

at high concentration

every intestine segments cunmlative absorption of punicalagins is (0.60±0.10)

(0.64±0.59)

(0.71±0.13)

(0.73±0.11) μg·cm-2. Conclusion: Absorption of punicalagins didn't be a special ‘absorption window’

punicalagins intestinal absorption followed the Zero-order kinetic equation.