Objective: To study the possible link between preventing effect of atherosclerosis (AS) and tight connection(TJ)Buyang Huanwu Tang(BYHWT). Method: Seventy-two male ApoE-/-mice were randomly divided into five groups:model group

three dose groups of BYHWT(5

10

20 g·kg-1·d-1)

Y-27632 group

and C57BL/6J mice was as a normal control group.AS model was established by intragastric administration of homocysteine(0.1 g·kg-1·d-1). Meanwhile

treatment groups were intragastrically administrated with BYHWT (5

10

20 g·kg-1·d-1)

Y-27632 50 mg·kg-1·d-1. Eight weeks later

Western-blot and reverse transcription PCR(RT-PCR) were used to determine the expression of aortic claudin

junction adhesion molecule (JAM)

zonula occludens (ZO). Result: Model group showed evident AS lesions.The expression of aortic claudin

JAM

ZO in the model group obviously decreased

compared with normal control group.Different doses of BYHWT could prevent atherosclerosis and up-regulate the claudin

JAM

ZO expressions. Conclusion: BYHWT could obviously increase the claudin

occludin

JAM

ZO expressions. BYHWT could prevent atherosclerosis induced by homocysteine via increasing the tight junction proteins expression.