Objective: To investigate the protective effects and its mechanism of Chaihu Shugan San(CHSGS) on hepatic fibrosis in rats. Method: The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum to rats.Seventy Wistar rats were divided into seven groups:blank group

model group

CHSGS high-dose group

mediate-dose group and low-dose group

prevention group

colchicin contrast group. Except the black groups

the other groups were injected with pig's serum without inactivation into abdominal cavity

twice per week and 0.5 mL each time

for 10 weeks. The prevention group was injected the stomach with the medicines(CHSGS 6.3 g·kg-1·d-1

10 weeks)

the other treatment groups were treated as the prevention groups 6 weeks after modeling for 4 weeks. Then rats were randomly taken for decollation in six groups at the end of the 10th week:colchicin contrast group 0.5 mL·kg-1·d-1

CHSGS low-dose

mediate-dose and high-dose groups (3.5

6.3

12.6 g·kg-1·d-1).The changes of the serum biochemical indicator of the hepatic function and hepatic fibrosis were detected respectively. Result: Compared with model group

the serum content of alanine aminotransferase(ALT)

aspartate aminotransferase (AST)

r-glutamyl endopeptidase(GGT)

alkaline phosphatase(ALP) and typeⅠ

Ⅲ

Ⅳcollage

hyaluronic acid (HA)

laminin (LN) were depressed obviously in treatment groips

the content of hydroxyproline(HYP) in liver tissue was significantly lower in CHSGS mediate-dose group. Conclusion: Chaihu Shugan San has an effect on hepatic fibrosis model rats.