Objective: To study the effects of petroleum ether extract

n-butanol extract and Rubus spp seed oil from R. spp seed on lipid metabolism and lipid peroxidation of hyperlipidemia in mice. Method: One hundred and twenty mice were randomly divided into 12 groups:blank group

model group

positive control group

petroleum ether extract high dose group (800 mg·kg-1)

medium dose group (400 mg·kg-1) and low dose group (200 mg·kg-1)

n-butanol extract high dose group (600 mg·kg-1)

medium dose group (300 mg·kg-1) and low dose group (150 mg·kg-1)

R. spp seed oil high dose group (1 000 mg·kg-1)

medium dose group (500 mg·kg-1) and low dose group (250 mg·kg-1). The mice hyperlipidemia model was established by ig high fat emul

the contents of triglycerides (TG)

total cholesterol (TC)

high density lipoprotein cholesterol (HDL-C)

low density lipoprotein cholesterol (LDL-C)

malondialdehyde (MDA)

and superoxide dismutase(SOD)activity in serum

and the coefficients of liver

kidney and spleen were tested. Result: Low dose group of petroleum ether extract could significantly decrease the contents of TG

TC

LDL-C and MDA and the coefficients of liver and spleen

and increase the content of HDL-C and the activity of SOD (P<0.05). Medium and low dose groups of n-butanol extract could significantly decrease the contents of TG

TC

LDL-C and MDA and the coefficients of liver and spleen and increase the activities of SOD(P<0.05). Medium dose group of R. spp seed oil could significantly decrease the contents of TG

TC and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD(P<0.05). High dose group of R. spp seed oil could significantly decrease the contents of TG

TC and MDA and the coefficients of liver and spleen

and increase the activity of SOD (P<0.05). High dose group of n-butanol extract could significantly decrease the contents of TG and MDA and the coefficients of liver and spleen and increase the content of HDL-C and the activity of SOD (P<0.05). Low dose group of R. spp seed oil could significantly decrease the contents of TG

TC

LDL-C and the coefficients of liver and spleen and increase the content of HDL-C (P<0.05). High (TG and TC) and medium (TC

HDL-C)

liver and spleen coefficients dose groups of petroleum ether extract were significant change. Compared with model group

other indicators had no significance

but there was a certain effect. Conclusion: Petroleum ether extract

n-butanol extract and R. spp seed oil had a certain degree of regulating lipid metabolism and lipid peroxidation. They could improve lipid accumulation in the liver and spleen of mice. It might be one of the possible mechanism of effective extracts of R. spp seed for reducing blood lipid.