人参花中总皂苷的提取工艺优选

孙乐; 李春梅; 郭迎迎; 吴巍; 刘淑莹

doi:10.13422/j.cnki.syfjx.2015010021

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人参花中总皂苷的提取工艺优选

工艺与制剂 | 更新时间：2024-01-04

- 人参花中总皂苷的提取工艺优选
- Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma
- 中国实验方剂学杂志 2015年21卷第1期页码：21-23
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省高校创新团队发展计划项目(JTD201213)
- DOI：10.13422/j.cnki.syfjx.2015010021
  中图分类号： R284.2
- 纸质出版日期：2015
- 稿件说明：
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孙乐, 李春梅, 郭迎迎, 等. 人参花中总皂苷的提取工艺优选[J]. 中国实验方剂学杂志, 2015,21(1):21-23.

SUN Le, LI Chun-mei, GUO Ying-ying, et al. Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 21-23.
孙乐, 李春梅, 郭迎迎, 等. 人参花中总皂苷的提取工艺优选[J]. 中国实验方剂学杂志, 2015,21(1):21-23. DOI： 10.13422/j.cnki.syfjx.2015010021.

SUN Le, LI Chun-mei, GUO Ying-ying, et al. Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 21-23. DOI： 10.13422/j.cnki.syfjx.2015010021.

摘要

目的: 优选人参花中总皂苷的提取工艺条件。方法: 采用UV测定总皂苷含量

以人参皂苷Re为指标成分

检测波长550 nm。以总皂苷提取量为指标

通过正交试验考察料液比、乙醇体积分数、提取时间和提取次数对人参花中总皂苷提取工艺的影响。结果: 总皂苷最佳提取工艺为料液比1:10

乙醇体积分数80%

提取温度85℃

提取时间1 h

提取数3次;总皂苷得率18.66%。结论: 优选的提取工艺稳定、合理

有效成分提取率高

为人参花资源的开发利用提供参考。

Abstract

Objective: To optimize extraction process of ginsenosides from flowers of Ginseng Radix et Rhizoma. Method: UV was adopted to determine the content of total saponins with ginsenoside Re as index ingredient and detection wavelength at 550 nm.Taking extracting amount of total saponins as index

orthogonal test was adopted to optimize extraction technology of ginsenosides with material-liquid ratio

ethanol concentration

extraction time and times as factors.The content of ginsenosides was determined by UV spectrophotometry. Result: Optimal extraction conditions were as follows:extracted thrice with 10 times the amount of 80% ethanol at 85℃

1 h for each time;yield of total saponins was 18.66%. Conclusion: This optimized extraction process is stable and reasonable with high yield of active constituents

it can provide a reference for development and utilization of flower resource of Ginseng Radix et Rhizoma.

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