Objective: To establish an ultra fast liquid chromatography (UFLC) method for simultaneous determination of alisol C 23-acetate

alisol A

alisol A 24-acetate

alisol G

alisol B and alisol B 23-acetate in the Alismatis Rhizoma. Method: The seperation was performed on an Ultimate UFLC-AQ C18 column (2.1 mm×100 mm

3 μm) using gradient elution with the mobile phase consisting of acetonitrile and water at a flow rate of 0.3 mL·min-1. The column temperature was maintained at 30℃

and the DAD detection wavelengths were set at 208 nm and 245 nm. Result: The linear range of alisol C 23-acetate

alisol A

alisol A 24-acetate

alisol G

alisol B and alisol B 23-acetate were 0.179 0-17.88(r=0.999 8)

0.500 0-100.0(r=0.999 7)

0.216 0-25.20 (r=0.999 6)

0.295 0-12.45 (r=1.000)

0.653 0-65.33 (r=0.999 6)

0.393 0-78.32 mg·L-1(r=0.999 8)

respectively. The average recoveries were 97.95%

96.70%

97.65%

96.01%

99.73%

100.30%. Conclusion: The results indicated that the developed method could be considered to be a simple

rapid and accurate method for the quality control of Alismatis Rhizoma.