近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量

白雁; 樊明月; 雷敬卫; 谢彩侠; 张强

doi:10.13422/j.cnki.syfjx.2015010073

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近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量

化学与分析 | 更新时间：2024-01-04

- 近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量
- Rapid Quantitative Determination of Ethanol-extract in Aurantii Fructus Immaturus by Near-infrared Spectroscopy
- 中国实验方剂学杂志 2015年21卷第1期页码：73-76
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省教育厅科学技术研究重点项目(14B360007);河南省中药开发工程技术研究中心项目(142102313109);河南中医学院科技创新人才项目(2014XCXRC03)
- DOI：10.13422/j.cnki.syfjx.2015010073
  中图分类号： O657.3;R284.1
- 纸质出版日期：2015
- 稿件说明：
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白雁, 樊明月, 雷敬卫, 等. 近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量[J]. 中国实验方剂学杂志, 2015,21(1):73-76.

BAI Yan, FAN Ming-yue, LEI Jing-wei, et al. Rapid Quantitative Determination of Ethanol-extract in Aurantii Fructus Immaturus by Near-infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 73-76.
白雁, 樊明月, 雷敬卫, 等. 近红外漫反射光谱法快速测定枳实药材中醇浸出物的含量[J]. 中国实验方剂学杂志, 2015,21(1):73-76. DOI： 10.13422/j.cnki.syfjx.2015010073.

BAI Yan, FAN Ming-yue, LEI Jing-wei, et al. Rapid Quantitative Determination of Ethanol-extract in Aurantii Fructus Immaturus by Near-infrared Spectroscopy[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 73-76. DOI： 10.13422/j.cnki.syfjx.2015010073.

摘要

目的: 采用近红外漫反射光谱技术结合偏最小二乘法(PLS)建立一种枳实药材中醇浸出物含量的快速测定方法。方法: 运用近红外漫反射光谱技术采集枳实的近红外漫反射光谱

以热浸法测定的含量为参考值

结合PLS建立枳实药材中醇浸出物含量的定量分析模型

并用未知样品验证该模型。结果: 所建醇浸出物定量分析模型的校正集内部交叉验证相关系数(R2)、内部交叉验证均方差(RMSECV)和校正均方差(RMSEC)分别为0.984 89

0.417 89和0.153;验证集预测相关系数(r)与预测均方差(RMSEP)分别为0.948 4和0.188。结论: 该方法操作简便

测定快速

结果准确

可用于枳实药材中醇浸出物含量的快速测定。

Abstract

Objective: This paper reported the application of near infrared spectroscopy with PLS for rapid quantitative determination of ethanol-extract in Aurantii Fructus Immaturus. Method: Through collecting near-infrared spectra of Aurantii Fructus Immaturus by near-infrared diffuse reflectance spectroscopy technique

with hot-dip method measurements as a reference value

the quantitative model of ethanol-extract was built by partial least squares (PLS)

and it was verified by the unknown samples. Result: The correlation coefficients (R2)

root mean square error of cross validation (RMSECV)

and the root mean-square error of calibration (RMSEC) of the quantitative calibration model of ethanol-extract was 0.984 89

0.417 89 and 0.153

respectively;the correlation coefficients of predication(r) and the root-mean-square error of predication (RMSEP) was 0.948 4 and 0.188

respectively. Conclusion: The method is simple

quick

accurate and pollution-free

and it can be applied for the rapid determination of ethanol-extract in Aurantii Fructus Immaturus.

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