Objective: To observe the differences of composition in volatile oil and anti-tumor effect in vitro within the different cultivated species and different processing products of Curcuma wenyujin. Method: The volatile oil was extracted by steam distillation

identified by GC-MS

then compared the inhibitive effect on cell line MGC-803 in vitro. Result: Although coming from the same Area

the different cultivated species and different processing products of C. wenyujin have obvious different inhibition on the growth of MGC-803 and have significantly when the volatile oil of C. wenyujin for high concentration

breed of fresh products inhibiting growth of MGC-803 was the highest was 20.2%. When the volatile oil of C. wenyujin for low concentration

common varieties of fresh products inhibiting growth of MGC-803 was the highest was 18.4%. Different chemical compositions in volatile oil. The chemical components are also obvious differences. Such as is beta.-phellandrene bicyclo[3.1.1]heptane

6

6-dimethyl-2-methylene

(1S)-is a characteristic component of traditional fresh product

cyclohexane

1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene )- is the characteristics of chemical composition of common fresh product

bicyclo[3.1.0]hex-2-ene

4-methyl-1-(1-methylethyl)

beta-pinene is the tissue culture of fresh products for characteristic components. Conclusion: Although coming from the same origin

the cultivated species and the processing method of C. wenyujin have great effects on the content and antitumorus activity of the volatile oil. The volatile oil of fresh and processed products breed the strongest inhibitory effect on MGC-803.