Objective: To investigate the spectrum-effect relationship of leaf extracts from Mangifera indica (LEMI) based on grey relational analysis. Method: High performance liquid chromatography (HPLC) was applied to establish the fingerprint chromatography of the leaf extracts extracted with different polar solvent. The anti-inflammatory effects of the extracts were observed via mice auricular swelling model induced by dimethylbenzene. Kunming mice were randomly divided into model group

dexamethasone positive group and LEMI groups. Model group was administered orally physiological saline;other groups were given corresponding drug for 5 days. Then ear swelling was determined. Then the grey relational analysis was used to investigate its spectrum-effect relationship. Result: There were significantly differences in the HPLC fingerprints and anti-inflammatory activities obtained from varied extracts. Compared with the model group

dexamethasone group had significantly inhibited the ear edema in mice (P<0.05). Chloroform extract and ethyl acetate LEMI from Red-ivory and Tainong-1 showed no significant anti-inflammatory effect

but other solvent LEMI had significant anti-inflammatory effect (P<0.05). 14 common peaks were detected in HPLC chromatography

which were used for the identification of herb products. It was found that the main anti-inflammatory effect was mostly contributed to X1

X3and mangiferin after grey relational analysis. Conclusion: The material basis of anti-inflammatory effect of LEMI were mangiferin

X1 and X3.Grey relational analysis was an effective and useful method to evaluate spectrum-effect relationship of Chinese herbs.