正交试验优化葛根中葛根素的闪式提取工艺

李宝红; 吴君; 程怡; 田圆; 王家平

doi:10.13422/j.cnki.syfjx.2015020027

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正交试验优化葛根中葛根素的闪式提取工艺

工艺与制剂 | 更新时间：2024-01-04

- 正交试验优化葛根中葛根素的闪式提取工艺
- Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test
- 中国实验方剂学杂志 2015年21卷第2期页码：27-29
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省自然科学基金项目(2012010009166)
- DOI：10.13422/j.cnki.syfjx.2015020027
  中图分类号： R284.2
- 纸质出版日期：2015
- 稿件说明：
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李宝红, 吴君, 程怡, 等. 正交试验优化葛根中葛根素的闪式提取工艺[J]. 中国实验方剂学杂志, 2015,21(2):27-29.

LI Bao-hong, WU Jun, CHENG Yi, et al. Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 27-29.
李宝红, 吴君, 程怡, 等. 正交试验优化葛根中葛根素的闪式提取工艺[J]. 中国实验方剂学杂志, 2015,21(2):27-29. DOI： 10.13422/j.cnki.syfjx.2015020027.

LI Bao-hong, WU Jun, CHENG Yi, et al. Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 27-29. DOI： 10.13422/j.cnki.syfjx.2015020027.

摘要

目的: 优选葛根中葛根素的闪式提取工艺。方法: 在单因素试验基础上

以葛根素提取率为评价指标

采用正交试验法考察乙醇体积分数、料液比、提取电压、提取时间对葛根中葛根素提取工艺的影响。采用HPLC测定葛根素含量

流动相甲醇-水(25:75)

检测波长250 nm。结果: 最佳提取工艺为加15倍量50%乙醇于90 V提取2 min;葛根素提取率37.42 mg ·g-1

转移率88.89%。结论: 优选的工艺稳定可行、重复性好、提取时间短

可作为葛根中葛根素的工业化生产工艺参数。

Abstract

Objective: To optimize flash extraction process of puerarin from Puerariae Lobatae Radix. Method: Based on single tests

taking extracting rate of puerarin as index

orthogonal test was adopted to optimize flash extraction process with ethanol concentration

solid-liquid ratio

extraction voltage and time as factors.HPLC was employed to determine the content of puerarin with mobile phase of methanol-water(25: 75) and detection wavelength at 250 nm. Result: Optimum extraction technology was as following:extracted 2 min with 15 times the amount of 50% ethanol at 90 V.Under these conditions

extracting rate of puerarin was 37.42 mg · g-1 and its transfer rate was 88.89%. Conclusion: This optimized process is stable and reproducible with short extracting time

it can be used as process parameters for industrial production of puerarin from Puerariae Lobatae Radix.

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