栀子中2个成分的分离鉴定及不同采收期栀子样品的比较分析

于晓蕾; 李艳芳; 苏瑞强; 赵志全

doi:10.13422/j.cnki.syfjx.2015020040

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栀子中2个成分的分离鉴定及不同采收期栀子样品的比较分析

化学与分析 | 更新时间：2024-01-04

- 栀子中2个成分的分离鉴定及不同采收期栀子样品的比较分析
- Isolation, Identification of Two Consitituents in Gardeniae Fructus and Their Quantitative Analysis in Different Harvesting Time
- 中国实验方剂学杂志 2015年21卷第2期页码：40-43
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重点基础研究发展计划(973计划)项目(2012CB724001)
- DOI：10.13422/j.cnki.syfjx.2015020040
  中图分类号： R284.1
- 纸质出版日期：2015
- 稿件说明：
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于晓蕾, 李艳芳, 苏瑞强, 等. 栀子中2个成分的分离鉴定及不同采收期栀子样品的比较分析[J]. 中国实验方剂学杂志, 2015,21(2):40-43.

YU Xiao-lei, LI Yan-fang, SU Rui-qiang, et al. Isolation, Identification of Two Consitituents in Gardeniae Fructus and Their Quantitative Analysis in Different Harvesting Time[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 40-43.
于晓蕾, 李艳芳, 苏瑞强, 等. 栀子中2个成分的分离鉴定及不同采收期栀子样品的比较分析[J]. 中国实验方剂学杂志, 2015,21(2):40-43. DOI： 10.13422/j.cnki.syfjx.2015020040.

YU Xiao-lei, LI Yan-fang, SU Rui-qiang, et al. Isolation, Identification of Two Consitituents in Gardeniae Fructus and Their Quantitative Analysis in Different Harvesting Time[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 40-43. DOI： 10.13422/j.cnki.syfjx.2015020040.

摘要

目的: 分离制备栀子药材中化学成分

建立栀子中该成分HPLC含量测定法。方法: 色谱条件采用Kromasil C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.1%磷酸水溶液(18:82)为流动相

流速1.0 mL ·min-1

检测波长327 nm

柱温40 ℃。结果: 从栀子药材中分离鉴定出6"-对香豆酰京尼平龙胆双糖苷(1)

5-二-O-咖啡酰基-4-O-(3-羟基-3-甲基)戊二酰基奎宁酸(2)2个成分;这2个成分在0.118 8～1.188

0.122 8～1.228 μg线性关系良好;平均回收率分别为99.23%

100.4%

RSD分别为2.4%

2.3%;栀子不同采收期对2种成分含量影响较大。结论: 本试验结果能较好地反映栀子不同采收期成分的变化

可为栀子药材质量标准的完善提供参考。

Abstract

Objective: To prepare chemical constituents and establish an HPLC method for determining these constituents in Gardeniae Fructus. Method: The chromatographic separations were obtained on a Kromasil C18 column (4.6 mm×250 mm

5 μm) with the mobile phase consisting of acetonitrile and 0.1% phosphoric acid aqueous solution (18: 82) at a flow rate of 1.0 mL · min-1. The detection wavelength was set at 327 nm

and the column temperature was 40 ℃. Result: 6"-p-coumaroyl genipin gentiobioside(1) and 3

5-di-O-caffeoyl-4-O-(3-hydroxy-3-methyl) glutaroyl quinic acid(2) were isolated from Gardeniae Fructus. These two constituents showed good linear relationship in range of 0.118 8-1.188 μg and 0.122 8-1.228 μg

respectively. The average recoveries were 99.23%(RSD 2.4%) and 100.4%(RSD 2.3%). Harvesting time has significant effect on the contents of the two constituents. Conclusion: The tests reflect the variation of the constituents in different harvesting time and the methods established are suitable for the quality improvement of Gardeniae Fructus.

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