Objective: To study the chemical constituents from Paeonia anomala and to evaluate their antioxidant activities. Method: The compounds were isolated by chromatography on silicagel

Sephadex LH-20 column and identified on the basis of physicochemical constants and spectral analysis. Their antioxidant activities were evaluated by 1

1-diphenyl-2-pieryl-2-picrylhydrazyl(DPPH) and 2

2-amino-di(3-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) radical scavenging methods. Result: Five compounds were isolated and their structures were identified as ethyl gallate (1)

methyl gallate (2)

oleanic acid (3)

syringate (4) and β-sitosterol(5). The results showed that the DPPH radical scavenging activity of compounds 1 and 2 were lower than the positive control of Vitamin C with IC50values (35.13±0.28)

(44.64±0.37) μmol · L-1. Their ABTS radical scavenging activity were lower than Vitamin C with IC50values of (25.13±0.68)

(35.76±1.48) μmol · L-1

respectively. Conclusion: Compounds 3

4 are isolated from this plant for the first time. Compounds 1

2 have powerful antioxidant activies

and the others have little antioxidant activies.