Objective: To study on the HPLC fingerprint of Scutellaria indica

establishing a method for the content determination of scutellarin

wogonside

apigenin and wogonin

simultaneously. Method: The separation was carried out on an Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm

5 μm) with the mixture of 0.6% acetic acid and acetonitrile and tetrahydrofuran as mobile phase in a gradient elution model (flow rate 1.0 mL · min-1

detection wavelength were 365 nm from 0 to 40 minute and 275 nm from 40 to 65 minute

column temperature 30 ℃

sample size 10 μL). 16 batches of S. indica were analyzed with the developed HPLC fingerprint and determination methods

the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A). Result: The linear range of scutellarin

wogonside

apigenin and wogonin were 5.125-82.00(r=0.999 9)

7.375-118.0 (r=0.999 9)

1.550-24.80(r=0.999 9)

7.188-115.0(r=0.999 9) mg · L-1

respectively. The average recovery rates of scutellarin

wogonside

apigenin and wogonin were 103.37%(RSD 1.8%)

100.63%(RSD 1.3%)

104.03%(RSD 0.8%)

98.39%(RSD 2.5%)

respectively. In the fingerprint

28 common peaks of S. indica were marked and 4 peaks were determined. Conclusion: This method provides a scientific basis for controlling the quality of S. indica.