HPLC测定温肾降浊散中大黄酸、大黄素及大黄酚的含量

方衡; 邹韬博; 刘海洋; 刘斌; 王发善; 张宁

doi:10.13422/j.cnki.syfjx.2015020091

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HPLC测定温肾降浊散中大黄酸、大黄素及大黄酚的含量

化学与分析 | 更新时间：2024-01-04

- HPLC测定温肾降浊散中大黄酸、大黄素及大黄酚的含量
- Simultaneous Determination of Rhein, Emodin and Chrysophanol in Wenshen Jiangzhuo Powder by HPLC
- 中国实验方剂学杂志 2015年21卷第2期页码：91-93
- 作者机构：
- 作者简介：
- 基金信息：
  
  黑龙江省自然科学基金面上项目(H201328);黑龙江省教育厅基金项目(12541758);黑龙江中医药大学优秀创新人才支持计划
- DOI：10.13422/j.cnki.syfjx.2015020091
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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方衡, 邹韬博, 刘海洋, 等. HPLC测定温肾降浊散中大黄酸、大黄素及大黄酚的含量[J]. 中国实验方剂学杂志, 2015,21(2):91-93.

FANG Heng, ZOU Tao-bo, LIU Hai-yang, et al. Simultaneous Determination of Rhein, Emodin and Chrysophanol in Wenshen Jiangzhuo Powder by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 91-93.
方衡, 邹韬博, 刘海洋, 等. HPLC测定温肾降浊散中大黄酸、大黄素及大黄酚的含量[J]. 中国实验方剂学杂志, 2015,21(2):91-93. DOI： 10.13422/j.cnki.syfjx.2015020091.

FANG Heng, ZOU Tao-bo, LIU Hai-yang, et al. Simultaneous Determination of Rhein, Emodin and Chrysophanol in Wenshen Jiangzhuo Powder by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 91-93. DOI： 10.13422/j.cnki.syfjx.2015020091.

摘要

目的: 建立温肾降浊散中大黄酸、大黄素及大黄酚的含量测定方法。方法: 采用高效色谱法

ANGLA Promosil C18色谱柱(4.6 mm×250 mm

5 μm)

柱温30 ℃

流动相乙腈-0.1%磷酸水梯度洗脱

流速1.0 mL ·min-1

检测波长224 nm

进样量10 μL。结果: 在上述色谱条件下

大黄酸、大黄素及大黄酚之间有良好的分离度

大黄酸线性范围 0.757 2~ 3.786 0 μg(r=0.999 4)

大黄素线性范围0.347 6~1.738 0 μg(r=0.999 3)

大黄酚线性范围1.731~8.656 μg(r=0.999 2)

平均回收率分别为101.7%

102.6%

101.1%

RSD分别为2.9%

2.3%

1.9%。结论: 该方法简便、准确

重复性好

可为温肾降浊散的质量控制提供可借鉴的分析方法。

Abstract

Objective: To simultaneous determine the contents of rhein

emodin and chrysophanol in Wenshen Jiangzhuo powder by HPLC. Method: An external standard method by HPLC with ANGLA Promosil C18 column (4.6 mm×250 mm

5 μm) was used with the column temperature was 30 ℃;the detection wavelength was 224 nm

and the injection volume was 10 μL. The flow rate was set at 1.0 mL · min-1 and gradient elution with acetonitrile and H2O (included 0.1% phosphoric acid). Result: Rhein

emodin and chrysophanol were well separated by this method. Linearities of rhein

emodin and chrysophanol were good in the ranges of 0.757 2-3.786 0 μg(r=0.999 4)

0.347 6-1.738 0 μg(r=0.999 3)

and 1.731-8.656 μg(r=0.999 2)

respectively. The average recoveries of rhein

emodin and chrysophanol were 101.7%

102.6% and 101.1%

RSD were 3.0%

2.3%

1.9%. Conclusion: The method is simple

accurate and reproducible

and can be used for quality control of Wenshen Jiangzhuo powder.

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