Objective: To investigate influence of different dose of Chuanxiong Rhizoma(LC) on pharmacokinetics of gastrodin(GAS) and gastrodigenin(HBA) from Gastrodiae Rhizoma(GE) in migraine model rats. Method: Rats were randomly divided into LC-GE(1: 3) group(doses of LC and GE extracts were 0.163

0.484 g · kg-1)

LC-GE(2:3) group(doses of LC and GE extracts were 0.326

0.484 g · kg-1) and LC-GE(4:3) group(doses of LC and GE extracts were 0.652

0.484 g · kg-1)

plasma and brain tissue was collected at different times after drug administration.Concentrations of GAS and HBA were determined by HPLC

a mixture of acetonitrile-0.01% phosphoric acid(2: 98) was employed as mobile phase for determination of GAS and HBA in rats plasma with temperature of column oven was maintained at 35 ℃.While mobile phase of acetonitrile-water(3: 97) was used for quantification of HBA in rats brain

column temperature was heated at 30 ℃.DAS 2.0 software was used to calculate pharmacokinetic parameters. Result: Area under curve[AUC(0-t)] of GAS in rats plasma from these three groups were (1 984.41±89.91)

(1 708.74±108.96)

(1 862.76±82.11) mg · L-1 · min

their mean residence time[MRT(0-t)] were (63.62±2.04)

(61.69±2.40)

(75.04±4.29) min

their clearance(CL) were (0.034 3±0.002 4)

(0.039 9±0.002 3)

(0.031 6±0.003 2) L · min-1 · kg-1

respectively.AUC(0-t) of HBA in rats brain from these three groups were (72 891.70±12 075.24)

(64 201.77±5 921.88)

(97 046.82±11 002.49) μg · L-1 · min

their MRT(0-t) were (82.15±15.34)

(68.33±11.22)

(96.68±8.87) min

their CL were (0.395 5±0.072 8)

(0.450 7±0.049 2)

(0.301 2±0.036 7) L · min-1 · kg-1

respectively. Conclusion: These findings demonstrate LC-GE(4: 3) group(equivalent to crude herbs ratio of 4: 1) can enhance bioavailability

prolong MRT and reduce elimination speed of GAS in plasma as well as HBA in brain by comparing with other compatibility groups

which is consistent with usual dose in ancient prescriptions.This study demonstrates compatibility connotation at a right dose-ratio of LC and GE through angle of pharmacokinetics.