Objective: To observe the effect of Chaihu Shugan decoction (CHSGD) on the content of serotonin(5-HT)

interleukin-1beta(IL-1β)

interleukin-6(IL-6)mRNA expression of hippocampus in chronic temporal lobe epilepsy and depression model rats. Method: The intraperitoneal injection of lithium chloride-pilocarpine was used to make epileptic-depression model

the modeling rats were randomly divided into model group

citalopram group

CHSGD-high

medium

low dose group and normal group. The drug intervention group was given citalopram 10 mg · kg-1 · d-1

CHSGD-H

M

L at dose of 10

5

2.5 g · kg-1 · d-1 orally

normal group and model group were given equal salineby continuous lavage for 4 weeks

twice each day. By forced swim test (FST) and the changes of sucrose consumption the depressive behavior was assessed

video was usec to monitor seizures

hippocampal 5-HT number was determined by liquid chromatography mass spectrometry. Hippocampal IL-1β

IL-6 mRNA expression was detected by real-time fluorescence quantitative PCR detection. Result: Compared with normal group

model group rats FST total fixed time extended obviously

the sugar water consumption decreased significantly

IL-1β mRNA expression quantity increased obviously (P<0.01);5-HT content reduced

IL-6 mRNA expression increased (P<0.05). Compared with model group

drug intervention after 4 weeks

citalopram

CHSGT-H

M dose group of seizures in rats reduced significantly

FST total fixed time shortened significantly

sugar water consumption increased significantly

the hippocampus IL-1β

IL-6 mRNA expression lowered significantly (P<0.01)

the content of 5-HT increase (P<0.05). Citalopram group and CHSGD-H

M dose group of comparison

there was no statistical significance. Conclusion: High

medium dose of Chaihu Shugan decoction can inhibit epilepsy comorbidity of depression in hippocampus of modle rats IL-1β

IL-6 mRNA expression

promote the expression of 5-HT

reduce the number of seizures and improve depressive behavior in rats.