Objective: To optimize purification technology conditions of total alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 macroporous resin. Method: Taking matrine as index component

UV was employed to determine the content of total alkaloids with detection wavelength of 415 nm.With the content of total alkaloids as index

effects of the concentration of sample solution

amount and type of eluent

elution velocity on purification process were investigated by single factor tests. Result: Optimum technology conditions were as follows:adjusted pH of sample solution to 3-4 with with hydrochloric acid

adjusted pH to 10 with 20% ammonia water

the concentration of sample solution 0.8 g·mL-1

sample flow rate of 2 BV·h-1

sample volume(equivalent as crude drug)-resin volume ratio of 1: 2.5

washed impurity with 2 BV of water

with 4 BV of 70% ethanol as eluant

elution flow rate of 4 BV·h-1.Purity of total alkaloids was 66.83%. Conclusion: D101 macroporous resin has good purification effect for total alkaloids from Sophorae Tonkinensis Radix et Rhizoma

optimized process is simple and feasible

this study provides a reference for resource utilization of Sophorae Tonkinensis Radix et Rhizoma.