Objective: To optimize flash extraction process of total polysaccharides from Solanum nigrum. Method: Phenol-sulfuric acid method was used to determine the content of total polysaccharides with detection wavelength at 490 nm.With extracting amount of total polysaccharides and yield of extract as comprehensive index

effects of extraction voltage

extraction time

extraction times and solid-liquid ratio on flash extraction process were investigated by orthogonal test.Optimum flash extraction process was compared with hot reflux method and ultrasonic method. Result: Optimum flash extraction process was adding 30 times the amount of water at 80 V and extracting 2 times for 2 min of each time.Extracting amounts of total polysaccharides were 3.760

3.232

4.585 mg·g-1 by hot reflux extraction

ultrasonic and flash extraction method

respectively. Conclusion: Extraction efficiency of flash extraction method is significantly higher than the other two kinds of methods

which is suitable for extracting total polysaccharide from S. nigrum

it is feasible and rapid with good application prospects.