Objective: To optimize purification process of total flavonoids from Pyrrosiae Folium by macroporous resin. Method: The content of total flavonoids was determined by UV-visible spectrophotometry

detection wavelength was 510 nm.With the content of total flavonoids as index

types of macroporous resin were screened by static adsorption-elution test

single factor tests were adopted to optimize purification technology with sample volume

ratio of diameter to height

the amount of eluent and so on. Result: D101 macroporous resin showed better adsorption-elution effect than others

optimum purification technology was as following:height-diameter ratio of 6-10

ratio of resin to crude drug 1: 2.73

the concentration of sample solution 0.5 g·mL-1

absorption velocity 2 BV·h-1

washed impurity with 8 BV of water

then eluted with 5 BV of 60% ethanol at 4 BV·h-1

collected eluent.Under these conditions

purity of total flavonoids was 59.06%. Conclusion: D101 macroporous resin has good purification effects on total flavonoids from Pyrrosiae Folium

this optimized purification technology is reasonable

stable and feasible.