Objective: To establish an HPLC method for determination of paeoniflorin and tanshinone ⅡA in Shujing Fangtong capsules. Method: Chromatographic test on paeoniflorin was performed on Luna 5u C18 column(4.6 mm×250 mm

5 μm)

mobile phase consisted of acetonitrile-0.05% monopotassium phosphate(15: 85)

detection wavelength was 230 nm

column temperature was 25 ℃

injection volume was 10 μL

flow rate was 1.0 mL·min-1.Similarly

chromatographic test on tanshinone ⅡA was performed on Diamonsil C18 column (4.6 mm×250 mm

5 μm)

mobile phase was methanol-water(80: 20)

detection wavelength was 270 nm

column temperature was 25 ℃

injection volume was 20 μL and flow rate was 1.0 mL·min-1. Result: Linear ranges of paeoniflorin and tanshinone ⅡA were 0.020 4-0.204

0.043 2-0.864 μg

average recoveries of them were 99.89%(RSD 1.5%) and 100.43%(RSD 1.1%)

respectively.RSDs of repeatability

stability and precidion test were less than 2%. Conclusion: This method is simple

accurate with good repeatability

which is suitable for quality control of Shujing Fangtong capsules.