Objective: To develop an HPLC method for simultaneous determination of five components (paeoniflorin

albiflorin

hyperin

rutin

isoquercitrin) in Yushu tablets. Method: An Agilent Zorbax SB-C18 (4.6 mm×250 mm

5 μm) was adopted. The mobile phase was 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B) with gradient elution. Analysis were performed at 25 ℃ with a flow rate of 0.8 mL·min-1

and the detection wavelength was 203 nm. Result: The method had a good linearity in the ranges of 0.360 5-0.036 0 μg (r=0.999 9) for albiflorin

1.005 8-0.100 6 μg (r=0.999 8) for paeoniflorin

0.150 7-0.015 1 μg (r=0.999 8) for rutin

0.091 9-0.009 2 μg (r=0.999 8) for hyperin

0.063 7-0.006 4 μg (r=1.000 0) for isoquercitrin. The average recoveries of five components were 100.51% (RSD 2.0%)

99.81% (RSD 2.0%)

99.09%(RSD 1.7%)

99.23%(RSD 1.8%)

and 99.65%(RSD 2.0%)

respectively. Conclusion: The method is dependable

simple and practical

and may be used to control the quality of Yushu tablets.