Objective: To screen urine biomarkers of rats after administrated aconitine and find out its related metabolic pathways. Method: Wister rats were divided into two groups which corresponded to control group(CG) and medication administration team (MAT)

respectively.In each group

there were five male rats and five female rats.Biological samples were handled by liquid-liquid extraction method.Ultra high performance liquid chromatography (UPLC) coupled with time of flight mass spectrometry (TOF-MS) was applied

positive and negative ion mode was used to monitor endogenous metabolites in urine samples of rats

principal component analysis was applied to distinguish biological samples

biomarkers were selected by partial least square discriminant analysis and found out related metabolic pathways of aconitine. Result: Four potential biomarkers were identified

which were propionylglycine methyl ester

Cys-Ser-Met

pyrrole-2-carboxylic acid and 5

8-dihydroxy-3

4-dihydrocarbostyril. Conclusion: This study indicates that analgesia of aconitine may be associated with reduction of serotonin secretion

which can provide research basis for further study on mechanism of aconitine poisoning.