Objective: To investigate the mechanism of Shenling Baizhu San (SLBZS) in treating dextra sulfate sodium (DSS) -induced inflammatory bowel disease (IBD) mice. Method: Eighty SPF BALB/c mice were randomly divided into control group

model group

positive group

low-

medium- and high-dose SLBZS groups. The IBD mice were fed 5% DSS in their drinking water for 7 days

and control mice received only water. The mice in SLBZS groups were given SLBZS at doses of 3

6

12 g·kg-1

the mice in positive group were given mesalazine sustained release granules at dose of 2 g·kg-1

and the control mice were given the same volume of normal saline by intragastric administration. The mice weight

stool occult blood in the stool

the calculation of disease activity (DAI)

pathological examination of intestinal mucosal lesions integral were observed after 15 days. The expression of Claudin protein was tested by Westorn bolt. The expression of Occluding

Claudin

ZO-1 and JAM mRNA were analyzed by RT-PCR. Result: The lesion was obvious

Clandin protein decreased obviously in the model group as compared with the control group (P<0.05). Claudin protein

Occluding

Claudin

ZO-1 and JAM mRNA increased in DSS and all doses SLBZS groups as compared with the control group (P<0.05). Conclusion: SLBZS has good effect in DSS-induced IBD mice by the regulation of intestinal epithelial cell tight junctions.