Objective: To study the effect of cytokines of polyporus polysaccharide(PPS)on lipopolysaccharide (LPS)-stimulated J774

and to explore its anti-inflammatory mechanism. Method: J774 cells were inoculated in 6-well plates with the density of 2×105 per well.The experiment was divided into blank group

model group

LPS plus low-dose PPS group(1 mg·L-1)

LPS plus middle-dose PPS group(10 mg·L-1) and LPS plus high-dose PPS group(100 mg·L-1) were set up in this study.Each group had six wells.J774 cells were polarized to inflammatory macrophage by treating with 10 mg·L-1 LPS for 3 h.Meanwhile

LPS-stimulated J774 cells were treated with PPS of 1

10

100 mg·L-1 for 3 h.The expressions of interleukin-1β(IL-1β)

interleukin-10(IL-10)

tumor necrosis factor-α(TNF-α)

interferon-γ(IFN-γ) and interleukin-6(IL-6) mRNA were detected by real-time quantitative PCR (RT-PCR).And the expressions of p38 mitogen-activated protein kinase(p38)

extracellular signal-regulated kinase(ERK42/44)

p65 mitogen-activated protein kinase (p65) protein were determined by using Western blotting. Result: Compared to blank group

the percentages of IL-1β

IL-10

TNF-α

IFN-γ and IL-6 mRNA of model group were significantly higher(P<0.01).It meant the inflammation model was successfully established.After treated with PPS

the expressions of IL-1β

IL-10

TNF-α

IFN-γ and IL-6 mRNA were reduced.And the percentages of p38

ERK42/44

p65 protein were decreased(P<0.01

P<0.05). Conclusion: PPS could reduce inflammation in LPS-stimulated J774 cells.It might be achived through the mitogen activated protein kinase(MAPK) pathway to reduce inflammation damage.