苦荞麦总黄酮对软脂酸诱导EA.hy926细胞PI3K合成的影响

赵丹玉; 李刚; 王艳杰; 冯晓帆; 杨雪峰; 柳春

doi:10.13422/j.cnki.syfjx.2015030169

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苦荞麦总黄酮对软脂酸诱导EA.hy926细胞PI3K合成的影响

药理 | 更新时间：2024-01-04

- 苦荞麦总黄酮对软脂酸诱导EA.hy926细胞PI3K合成的影响
- Effect of Total Flavonoids of Tartary Buckwheat on Palmitic Acid-induced PI3K Synthesis in EA.hy926 Cells
- 中国实验方剂学杂志 2015年21卷第3期页码：169-171
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省杰出青年学者成长计划项目(LJQ2013102);辽宁省博士科研启动基金项目(20121100);辽宁省社发攻关及成果产业化项目(2013020195-202)
- DOI：10.13422/j.cnki.syfjx.2015030169
  中图分类号： R285
- 纸质出版日期：2015
- 稿件说明：
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赵丹玉, 李刚, 王艳杰, 等. 苦荞麦总黄酮对软脂酸诱导EA.hy926细胞PI3K合成的影响[J]. 中国实验方剂学杂志, 2015,21(3):169-171.

ZHAO Dan-yu, LI Gang, WANG Yan-jie, et al. Effect of Total Flavonoids of Tartary Buckwheat on Palmitic Acid-induced PI3K Synthesis in EA.hy926 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 169-171.
赵丹玉, 李刚, 王艳杰, 等. 苦荞麦总黄酮对软脂酸诱导EA.hy926细胞PI3K合成的影响[J]. 中国实验方剂学杂志, 2015,21(3):169-171. DOI： 10.13422/j.cnki.syfjx.2015030169.

ZHAO Dan-yu, LI Gang, WANG Yan-jie, et al. Effect of Total Flavonoids of Tartary Buckwheat on Palmitic Acid-induced PI3K Synthesis in EA.hy926 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 169-171. DOI： 10.13422/j.cnki.syfjx.2015030169.

摘要

目的: 研究苦荞麦总黄酮对于软脂酸诱导EA.hy926细胞PI3K合成的影响。方法: 将体外培养的EA.hy926细胞分为正常组、模型组、苦荞麦总黄酮组及二甲双胍组。采用RT-PCR以及免疫细胞化学法分别测定各组细胞PI3K mRNA和蛋白的表达。结果: 模型组细胞PI3K mRNA和蛋白表达与正常组相比明显下降(P<0.01)。苦荞麦总黄酮组与二甲双胍组细胞PI3K mRNA和蛋白表达与模型组相比明显增加(P<0.01)。治疗组间无明显差异。结论: 苦荞麦总黄酮对于软脂酸诱导下EA.hy926细胞PI3K合成具有明显促进作用。

Abstract

Objective: To observe the effects of total flavonoids of tartary buckwheat on palmitic acid-induced PI3K in EA.hy926 cells. Method: EA.hy 926 cells were cultured in vitro and divided into the control group

the model group

the total flavonoids of tartary buckwheat group and the metformin group. The PI3K mRNA and protein expression levels were determined by RT-PCR and immunocytochemistry

respectively. Result: Compared with the control group

the expression levels of PI3K mRNA and protein were significantly lower in the control group (P<0.01). Compared with the control group

the PI3K mRNA and protein expression increased markedly in both total flavonoids of tartary buckwheat group and metformin group (P<0.01)

and there is no significant difference between two groups. Conclusion: Total flavonoids of tartary buckwheat could effectively promote the expression of PI3K mRNA and protein in endothelial cells under palmitic acid stimulation.

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