正交试验优选复方肠泰颗粒提取工艺

石慧慧; 李萍; 戴运好; 鞠建明; 王璐璐; 霍介格

doi:10.13422/j.cnki.syfjx.2015040023

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正交试验优选复方肠泰颗粒提取工艺

药剂与炮制 | 更新时间：2024-01-04

- 正交试验优选复方肠泰颗粒提取工艺
- Optimization of Extraction Process of Compound Changtai Granules by Orthogonal Design
- 中国实验方剂学杂志 2015年21卷第4期页码：23-26
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省中医药研究院医院制剂开发专项(Y1201)
- DOI：10.13422/j.cnki.syfjx.2015040023
  中图分类号： R286.0
- 纸质出版日期：2015
- 稿件说明：
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石慧慧, 李萍, 戴运好, 等. 正交试验优选复方肠泰颗粒提取工艺[J]. 中国实验方剂学杂志, 2015,21(4):23-26.

SHI Hui-hui, LI Ping, DAI Yun-hao, et al. Optimization of Extraction Process of Compound Changtai Granules by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 23-26.
石慧慧, 李萍, 戴运好, 等. 正交试验优选复方肠泰颗粒提取工艺[J]. 中国实验方剂学杂志, 2015,21(4):23-26. DOI： 10.13422/j.cnki.syfjx.2015040023.

SHI Hui-hui, LI Ping, DAI Yun-hao, et al. Optimization of Extraction Process of Compound Changtai Granules by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 23-26. DOI： 10.13422/j.cnki.syfjx.2015040023.

摘要

目的: 优选复方肠泰颗粒的提取工艺

为该制剂的工业化生产提供参考。方法: 以浸出物、人参皂苷Rb1及常春藤皂苷元得率为综合评价指标

采用L9(34)正交试验考察加水量、提取时间和煎煮次数对提取效果的影响。采用HPLC测定人参皂苷Rb1和常春藤皂苷元含量

流动相分别为流动相乙腈(A)-水(B)梯度洗脱(0~5 min

10%~30%A;5~20 min

30%~45%A;20~22 min

45%~10%A)和乙腈-0.1%甲酸水溶液(70:30)。结果: 最佳提取工艺为分别加10

8倍量水提取3次

每次1 h。人参皂苷Rb1和常春藤皂苷元质量分数分别为2.48

10.50 mg ·g-1

浸出物得率16.72%。结论: 优选的提取工艺重复性好、稳定可行

适用于复方肠泰颗粒的工业化生产。

Abstract

Objective: To optimize extraction technology of compound Changtai granules for industrial production of this preparation. Method: Orthogonal test was employed to investigate influences of the amount of water

extracting time and decoction times on extraction process by taking contents of ginsenoside Rb1

hederagenin and extract as comprehensive evaluation indexes.Contents of ginsenoside Rb1 and hederagenin were determined by HPLC

mobile phases were consisted of acetonitrile(A)-water(B) for gradient elution(0-5 min

10%-30%A;5-20 min

30%-45%A;20-22 min

45%-10%A) and acetonitrile-0.1% aqueous formic acid(70: 30). Result: Optimum extraction technology was:refluxing extracted 3 times with 10

8 times the amount of water with 1 h per time.Under these conditions

contents of ginsenoside Rb1 and hederagenin were 2.48

10.50 mg · g-1

respectively;yield of extract was 16.72%. Conclusion: This optimized extraction technology is feasible

stable and suitable for industrial production of compound Changtai granules.

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