Objective: To establish HPLC fingerprints of water-soluble components in Atractylodis Rhizoma and determine the content of atractyloside A. Method: HPLC was adopted to determine the content of atractyloside A with mobile phase of acetonitrile(A)-0.25% aqueous formic acid(B) for gradient elution(0-17 min

2%-5%A;17-25 min

5%-12%A;25-35 min

12%A;35-50 min

12%-17%A;50-70 min

17%-34%A;70-80 min

34%-40%A)

detection wavelength at 221 nm and flow rate of 0.6 mL · min-1.The external standard method was employed to determine the content of atractyloside A in ten batches of Atractylodis Rhizoma. Result: Fingerprint of water extract in Atractylodis Rhizoma was established by HPLC

and 18 common peaks were detected.RSD of common peaks relative retention time and relative peak area in precision

stability and repeatability tests were less than 3%.Water extract of ten batches of samples showed high stability with the content of atractyloside A in Atractylodis Rhizoma was 2.775 mg · g-1. Conclusion: This established method is specific

accurate and reproducible

it can be used as a quality control method for Atractylodis Rhizoma.