Objective: To establish an HPLC method for simultaneous determination of baicalin

ginsenosides Rg1

ginsenoside Rb1

notoginsenoside R1 and special privet glycosides five in Qibai granules. Method: HPLC analysis was performed on a Prevail C18 column (4.6 mm×250 mm

5 μm) with methanol(A) -0.2% phosphoric acid (B) as mobile phase by gradient elution [0-5min

45%A

5-15 min

45%-50% A

15-60 min

50% A]. The flow rate was 1.0 mL ·min-1

column temperature was maintained at room temperature

and the detection wavelength was set at 203 nm. Result: The linear ranges of baicalin

ginsenosides Rg1

ginsenoside Rb1

notoginsenoside and special privet glycosides were 2.16-21.6

1.95-19.5

2.64-26.4

3.51-35.1 and 1.01-10.1 mg · L-1

respectively. The RSD of precision

reproducibility and stability tests were less than 2%

the average recoveries were ranging from 95.92% to 101.90%. Conclusion: This method is simple

reasonable

reliable and could be used for quality control of Qibai granules.