Objective: As the long chain hydrocarbons

punicic acid (C18: 3) on the structure of 9(Z)

11(E)

13(Z)-octadecatrienoic acid

which was in 200-400 nm absorption-free and can't be measured directly by UV detector. This experiment adopted column derivatization methods

made the punicic acid with a strong chromophore in maximum absorption in 200-400 nm. The determination method of punicicacid in pomegranate seed oil by HPLC was established in this thesis. Method: The punicic acid were derivatized with ω-bromoacetophenone as derivative reagent

triethanolamine as catalyst

the Diamonsi C18column(4.6 mm×250 mm

5 μm)was used. The detection wavelength was set at 272 nm

column temperature 30 ℃

with methanol-acetonitrile-water(68.5: 20: 11.5)aseluent

the flow rate was 1.0 mL · min-1. Result: The standard curves of punicic acid is linear within in the range of 0.026 6-0.133 0 g · L-1 and the coefficient is 0.999.The average recovery of punicic acid is 98.7%

RSD is 1.8%. Conclusion: The method is reproducible and accurate

which can be used as the determination method of punicic acid inpomegranate seed oil

and used for the quality control of pomegranate seed oil.