XIN Yang, ZHANG Ying, ZHANG Zhe, et al. Analysis on Metabolites of Hypaconitine in Rats' Urine by LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 94-97.
XIN Yang, ZHANG Ying, ZHANG Zhe, et al. Analysis on Metabolites of Hypaconitine in Rats' Urine by LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 94-97. DOI: 10.13422/j.cnki.syfjx.2015040094.
Objective: To analyze metabolites of hypaconitine in rats' urine. Method: Urine samples of rats were purified by liquid-liquid extraction
they were detected by liquid chromatography coupled with ion trap mass spectrometry.HPLC was performed by applying gradient elution with mobile phase composed of buffer of 2% ammonia combined with 0.2% acetic acid (A)-methanol blended with acetonitrile of equal volume (B)-methanol(C). Result: Prototype (M1) and three metabolites (M2
M3
M4) of hypaconitine were detected
M2 was identified as 18-O-demethylhypaconitine
M3 was identified as N-demethylhypaconitine
M4 was identified as a hydroxylated metabolite. Conclusion: Hypaconitine may occur demethylation
hydroxylation metabolic reactions
which could provide a reference for further research on pharmacodynamics of hypaconitine.