Objective: To study the mechanism of Fufang Yuyang powder on the diabetic skin ulcers healing. Method: The SD male rats were given alloxan and skin was scratched to establish the diabetic wound model. The modeling rats were randomly divided into 5 groups:model group

positive control group(Kangfuxin prescription 10.0 g · kg-1

ig)

Fufang Yuyang powder group (Fufang Yuyang powder 13.0 g · kg-1

ig)

reinforce deficiency group (Buxu prescription 5.1 g ·kg-1

ig)

removing blood stasiss group (Quyu prescription 7.9 g · kg-1

ig). enzyme linked immunosorbent assay(ELISA) was used to assay type Ⅰ and Ⅲ collagen

Smad3

Smad7

transforming groowth factor(TGF)-beta at 10

16

20 days after modeling. Result: Compared with the model group

Fufang Yuyang powder increased Ⅲ type collagen

Smad7 expression(P<0.05)

decreased Smad3 expression(P<0.05)

on the 20th day

the action was most significant

Buxu prescription increasedⅠ type collagen expression (P<0.05)

increased TGF-β1

Smad3 expression(P<0.05);Quyu prescription increasedⅠ

Ⅲ type collagen expression(P<0.05)

increased

Smad7 expression(P<0.05). Kangfuxin group regulated Ⅲ collagen

increased TGF-β1

Smad3 expression(P<0.05). Conclusion: Fufang Yuyang powder can promote the wound healing in diabetes rats

the mechanism may be related to bidirectional regulation of TGF β1-Smad3

Smad7 pathway and adjusting of the proportion of Ⅰ

Ⅲ type collagen in wound tissue.