Objective: To investigate the protective effect of total flavonoids of Clerodendranthus spicatus on Parkinson disease (PD) in rat and cell models. Method: The PD model was induced by positioning injection of 6-hydroxydopamine on right substantia nigra of rats. Seventy PD rats were randomly divided into 5 groups:the model group;the high-

middle-

low-dose total flavonoids of C. spicatus groups (45

22

11 mg · kg-1 · d-1);the madopar group (7.8 mg · kg-1 · d-1). Another 10 healthy rats were assigned to the control group. After 14 days of treatment

the levels of malondialdehyde (MDA)

catalase (CAT)

glutathion peroxidase (GSH-Px)

superoxide dismutase (SOD)

dopamine (DA) and 4-hydroxy-3-methoxyphenylacetic (HVA) in brain tissues of rats were detected by ELISA. SH-SY5Y cells were divided into the control group

the model group

and different dosage of total flavonoids of C. spicatus groups (2

1

0.5

0.25 g · L-1). The PD cell model was induced by adding 30 μmol ·L-1 6-OHDA. The survival rate and morphology were observed and compared after 20-h incubation. Result: Compared with the control group

rotating frequency of rats in PD model group increased

the levels of CAT

GSH-Px

SOD and HVA in brain tissue decreased significantly

MDA level increased with statistically significant difference (P<0.01). Compared with the model group

rotated times of PD rats decreased significantly in the high-dose total flavonoids of C. spicatus group (P<0.05)

the levels of CAT

GSH-Px

SOD and HVA increased

MDA concentration decreased in all doses of total flavonoids of C. spicatus groups (P<0.05

P<0.01). However

the improvement of neurological behavior and DA level in the total flavonoids of C. spicatus groups were not as good as madopar. Moreover

the total flavonoids of C. spicatus(2

1 g · L-1) pretreatment could reduce the cellular damage caused by 6-OHDA as compared with the control group(P<0.05

P<0.01). Conclusion: Total flavonoids of C. spicatus have obvious protective effect on the PD rat model and cell model induced by 6-OHDA. The protective effects may be related to reducing cell damage by resisting oxidative stress.