影响酶解去除薏苡仁淀粉过程的关键参数考察

孙文杰; 陈彦; 瞿鼎; 孔悦; 刘聪燕; 周静; 袁成甜

doi:10.13422/j.cnki.syfjx.2015050016

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影响酶解去除薏苡仁淀粉过程的关键参数考察

药剂与炮制 | 更新时间：2024-01-04

- 影响酶解去除薏苡仁淀粉过程的关键参数考察
- Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis
- 中国实验方剂学杂志 2015年21卷第5期页码：16-19
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81373979);江苏省中医药领军人才项目(LJ200913);江苏省自然科学基金项目(BK20141058)
- DOI：10.13422/j.cnki.syfjx.2015050016
  中图分类号： R283.6
- 纸质出版日期：2015
- 稿件说明：
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孙文杰, 陈彦, 瞿鼎, 等. 影响酶解去除薏苡仁淀粉过程的关键参数考察[J]. 中国实验方剂学杂志, 2015,21(5):16-19.

SUN Wen-jie, CHEN Yan, QU Ding, et al. Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 16-19.
孙文杰, 陈彦, 瞿鼎, 等. 影响酶解去除薏苡仁淀粉过程的关键参数考察[J]. 中国实验方剂学杂志, 2015,21(5):16-19. DOI： 10.13422/j.cnki.syfjx.2015050016.

SUN Wen-jie, CHEN Yan, QU Ding, et al. Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 16-19. DOI： 10.13422/j.cnki.syfjx.2015050016.

摘要

目的: 考察影响高温α-淀粉酶酶解去除薏苡仁中淀粉的关键参数

为薏苡仁总多糖的应用提供参考。方法: 以还原糖含量及碘液检试样品颜色变化作为定量及定性指标

在单因素试验基础上

通过正交试验考察酶解温度、酶用量、酶解时间、酶解pH对薏苡仁中淀粉酶解程度的影响。结果: 最佳酶解参数为酶用量600 U·g-1

酶解温度75 ℃

酶解pH 6.0

酶解时间120 min;薏苡仁总多糖水提液中还原糖质量分数约25%

且碘试液检测不变色

薏苡仁总多糖纯度达76.63%

较未去除淀粉的薏苡仁总多糖纯度54.41%显著提高。结论: 高温α-淀粉酶可简单、快速的去除薏苡仁中淀粉

有利于高纯度薏苡仁总多糖的获得。

Abstract

Objective: To provide a reference for application of total polysaccharides in Coicis Semen by investigating effect of key parameters on high temperature α-amylase enzymolysis for starch in Coicis Semen. Method: Take the content of reducing sugar as a quantitative indicator and color change after iodine solution testing as a qualitative indicator

based on single factor tests

orthogonal test was adopted to investigate effects of the amount of enzyme

hydrolysis temperature

hydrolysis pH and reaction time on amylohydrolysis. Result: Optimal parameters were as follows:the amount of enzyme 600 U·g-1

enzymolysis temperature 75 ℃

enzymatic pH of 6.0

enzymolysis time of 120 min.The content of reducing sugar in extract of total polysaccharides in Coicis Semen was about 25%

meanwhile

color of samples did not alter after iodine solution testing.Purity of total polysaccharides in Coicis Semen was 76.63%

which showed a great improvement by comparing with 54.41% of samples without removing starch. Conclusion: High-temperature α-amylase can remove starch from Coicis Semen readily and rapidly

it is conducive to extraction of total polysaccharides.

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