Objective: To optimize extraction technology of Qizhu granules. Method: With contents of astragaloside and paeoniflorin as comprehensive evaluation index

orthogonal test was adopted to optimize water extracting technology.Taking composite score of extracting amounts of specnuezhenide and berberine hydrochloride as index

orthogonal test was adopted to optimize ethanol extraction process.The content of astragaloside was determined by HPLC-ELSD

mobile phase consisted of acetonitrile-water (34: 66).HPLC-DAD was employed to determine contents of paeoniflorin

specnuezhenide and berberine hydrochloride with mobile phases of methanol-0.05 mol·L-1 potassium dihydrogen phosphate solution (30: 70)

methanol-water (32: 68)

0.05 mol·L-1potassium dihydrogen phosphate solution-acetonitrile(50: 50) and detection wavelengths of 230

224

345 nm

respectively. Result: The best water extraction process was:extracted twice with 12

10 times the amount of water for 2 h per time.Optimum ethanol extraction process was as following:extracted thrice with 6 times the amount of 60% ethanol for 2 h each time. Conclusion: These optimized technologies are stable

feasible and suitable for standardized production of Qizhu granules.