HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量

张广仁; 吴云; 吴莎; 萧伟

doi:10.13422/j.cnki.syfjx.2015050065

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HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量

化学与分析 | 更新时间：2024-01-04

- HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量
- Simultaneous Determination of Five Compounds in Sanjie Zhentong Capsules by High-performance Liquid Chromatography Coupled with Ultraviolet and Evaporative Light Scattering Detectors
- 中国实验方剂学杂志 2015年21卷第5期页码：65-68
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"重大新药创制"科技重大专项(2011ZX09201-201-20)
- DOI：10.13422/j.cnki.syfjx.2015050065
  中图分类号： O657;R286.0
- 纸质出版日期：2015
- 稿件说明：
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张广仁, 吴云, 吴莎, 等. HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量[J]. 中国实验方剂学杂志, 2015,21(5):65-68.

ZHANG Guang-ren, WU Yun, WU Sha, et al. Simultaneous Determination of Five Compounds in Sanjie Zhentong Capsules by High-performance Liquid Chromatography Coupled with Ultraviolet and Evaporative Light Scattering Detectors[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 65-68.
张广仁, 吴云, 吴莎, 等. HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量[J]. 中国实验方剂学杂志, 2015,21(5):65-68. DOI： 10.13422/j.cnki.syfjx.2015050065.

ZHANG Guang-ren, WU Yun, WU Sha, et al. Simultaneous Determination of Five Compounds in Sanjie Zhentong Capsules by High-performance Liquid Chromatography Coupled with Ultraviolet and Evaporative Light Scattering Detectors[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 65-68. DOI： 10.13422/j.cnki.syfjx.2015050065.

摘要

目的: 建立HPLC-UV-ELSD同时测定散结镇痛胶囊中皂苷类和黄酮类成分的方法

比较15批散结镇痛胶囊中各成分含量。方法: 采用HPLC-UV-ELSD联用法

Waters Symmetry@C18色谱柱(4.6 mm×250 mm

5 μm)

乙腈(A)-水(B)梯度洗脱(1~13 min

19%~37%A;13~50 min

37%~36%A)

流速1 mL·min-1

检测波长278 nm;蒸发光散射检测器(ELSD)漂移管温度45 ℃

载气流速3.7 L·min-1

同时测定散结镇痛胶囊中三七皂苷R1

人参皂苷Rg1

人参皂苷Rb1

龙血素A和龙血素B的含量。结果: 建立了同时测定散结镇痛胶囊中3个皂苷类和2个黄酮类成分含量的方法

线性关系良好(0.999 5~0.999 7)

平均回收率为 99.1%~104.2%。结论: 该方法简便

重复性好

适用于散结镇痛胶囊皂苷类和黄酮类成分的同时测定

亦适用于散结镇痛胶囊的的质量控制。

Abstract

Objective: To establish a quantitative method of three main saponins and two major active flavonoids in Sanjie Zhentong capsules by high-performance liquid chromatography coupled with ultraviolet detectors and evaporative light scattering detectors (HPLC-UV-ELSD)

and compare the contents of notogin-senoside R1

ginsenoside Rg1

ginsenoside Rb1

loureirin A

loureirin B among 15 batches of the drugs. Method: HPLC-UV-ELSD was conducted on a Waters Symmetry@C18(4.6 mm×250 mm

5 μm) with gradient elution

the mobile phase was ACN and H2O

the temperature of drift tube was 45 ℃ and the nebulizing gas flow rate was 3.7 L·min-1. Result: Five compounds were simultaneously detected with one injection

good linear relationships were found(0.999 5-0.999 7)

and the average recovery rates were 99.1%-104.2%. Conclusion: The HPLC-UV-ELSD method is simple and reproducible

which can be used for quality control of Sanjie Zhentong capsules.

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