Objective: To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside

hypericin

rutin

isoquercetin

myricetin

quercetin

loganin

morroniide in compound Huangkui granules. Method: The determination was performed on an Aglient C18(4.6 mm×250 mm

5 μm) chromatographic column

the mobile phase was acetonitrile-0.05% formic acid aqueous solution with gradient elution at a flow rate of 1.0 mL·min-1;the detection wavelength was 240

260 and 360 nm

column temperature was 30 ℃. Result: The calibration curves of calycosin-7-O-β-D-glucoside

hypericin

rutin

isoquercetin

myricetin

quercetin

loganin

morroniide were linear in the ranges of 3.5-35.0(r=0.999 6)

32.09-300.9(r=0.999 7)

2.21-22.12(r=0.999 7)

18.68-186.9(r=0.999 8)

6.32-63.20(r=0.999 7)

3.54-35.44(r=0.999 6)

12.39-123.9(r=0.999 6)

12.88-128.8 mg·L-1(r=0.999 6)

respectively. The average recoveries were 98.1%(RSD 1.7%)

99.7%(RSD 0.6%)

98.7%(RSD 2.5%)

100.1%(RSD 1.4%)

99.7%(RSD 1.0%)

99.9%(RSD 2.7%)

99.0%(RSD 1.2%)

99.7%(RSD 1.3%)

respectively. Conclusion: The method established is simple

accurate and reproducible

which can be used for the quality control of Huangkui granules.