Objective: To observe the effect of Yishen Huazhuo decoction on express of brain derived neurotrophic factor(BDNF) and tyrosine kinase keceptor B(TrkB) in the hippocampus of experimental rat model with Alzheimer's disease and explore the mechanism of Yishen Huazhuo decoction. Method: Male SD rats were randomly divided into 5 groups

sham operation group

model group

low

middle and high dose group of Yishen Huazhuo decoction. According to the brain stereotaxic atlas of rats

animal model of Alzheimer's disease was established by injecting Aβ25-35 into the left lateral ventricle. Treatment groups were given three different doses of Yishen Huazhuo decoction (2.8

5.6

11.2 g·kg-1) within the next 4 weeks

once daily. The Morris Water Maze was employed to investigate the function of learning and memory in model rats. The effect of protein expression of BDNF and its TrkB receptor in the hippocampus were detected by means of Western blot. Result: Compared with sham operation group

models’ learning and memory ability and the protein expression of BDNF and its TrkB receptor in the hippocampus were significantly lower (P<0.05). Yishen Huazhuo decoction could significantly enhance the learning and memory ability and the protein expression of BDNF as well as its TrkB receptor in the hippocampus of the rats compared with the model group (P<0.05). Conclusion: Yishen Huazhuo decoction could promote the protein expression of BDNF and its TrkB receptor in the hippocampus

and improve the learning and memory ability of the AD rats.