Objective: To study chemical compositions changes of different extracting parts of Jiuwei Tongqiao decoctions by gradient extraction with four kinds of organic solvents

screen effective extracting parts by in vitro pharmacodynamic

select the best effective parts on proliferation effect of anti-glioma cells

and provide experimental basis for further study on mechanism of Jiuwei Tongqiao decoctions. Method: After alcohol extract enrichment of Jiuwei Tongqiao decoctions

gradient extraction with petroleum ether

chloroform

ethyl acetate and water saturated n-butyl alcohol was to get petroleum ether extract(part Ⅰ)

chloroform extract(part Ⅱ)

ethyl acetate extract(part Ⅲ)

water saturated n-butyl alcohol extract(part Ⅳ) and the residual liquid water extract(part Ⅴ).HPLC was employed to determined four index components

including calycosin-7-O-β-D-glucoside

formononetin-7-O-β-D-glucoside

calycosin and formononetin

mobile phase was consisted of (acetonitrile+0.05% formic acid)(A)-(water+0.05% formic acid)(B) for gradient elution (0-50 min

5%-55%A;50-70 min

55%-60%A;70-90 min

60%-85%A;90-100 min

85%-100%A) and detection wavelength was 254 nm.U251 cells of anthropogenic brain glioma was selected for tracking

MTT was applied to determine effect of extraction parts for cell survival. Result: The content ratio of calycosin glucoside-ononin-calycosin in part Ⅲ was 1.0: 10.8: 8.2

its half inhibition rate(IC50) was 46.89 g·L-1.In comparison with other parts

index ingredients in part Ⅲ were more comprehensive and concentration of them were higher

inhibitory effect on U251 cells was more obvious and appeared a certain concentration-dependent manner. Conclusion: Part Ⅲ is the most effective parts in Jiuwei Tongqiao decoctions for anti-proliferative effect of U251 glioma cells.