Objective: To analyze the toxicity source of Curculiginis Rhizoma and Epimedii Folium and to study the correlation between their toxicity and the content of curculigoside and icariin with several compatible prescriptions. Method: Under the total dosage being constant

several compatible prescriptions were obtained by uniform design with Curculiginis Rhizoma and Epimedii Folium classical proportion 1: 1 as a reference. Their extracts were obtained being by water and alcohol. Their toxicity was compared in mice. The contents of curculigoside and icariin in each compatible prescriptions were determineby HPLC

and the correlation between toxicity and content were analyzed. Result: The acute toxicity sequence is alcohol extract of Curculiginis Rhizoma-Epimedii Folium(1: 0)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium(2: 1)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (1: 1)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (1: 2)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (0: 1)>water extract group. There was significantly positive correlation between the content of curculigoside with their toxicity(r=0.967

P<0.01). No correlation was found between the content of Icariin and their toxicity (r=-0.053

P>0.05). Conclusion: Toxic components of Curculiginis Rhizoma and Epimedii Folium mainly come from Curculiginis Rhizoma

and they can be easily enriched in alcohol extracts. There is positive correlation between the toxicity and the content of curculigoside.