Objective: The aim of this article was to explore the acute toxicity of water extracts and alcohol extracts from stalks and leaves of Coix on mice so as to determine the median lethal dose (LD50) and the maximum tolerated dose (MTD). Method: Seventy-four KM mice (half male and half female) of grade SPF were divided into extract group and control group. After fasted for 16 hours before experiment

all the mice were fed by gavage. The toxic reaction and death were oberved. If the mice died

it will be dissected to observe the changes of heart

liver

spleen

lung

kidney

brain

stomach

small intestine and other organs by eyes.After the observation for 14 days

the survivals in each group were killed

and then dissected. Then we examined their main pathological changes in organs by eyes as mentioned above. Result: No obvious symptoms of poisoning were discovered on these mice

neither death. Conclusion: The maximum tolera dose of SPF KM mice to the water extracts and the alcohol extracts from leaves of Coix

and to the water extract and alcohol extracts from stalks of Coix

were 42.08

34.80

39.52

32.64 g·kg-1

respectively

with all greater than 5 g·kg-1. According to the quarter experiment

the water and alcohol extracts from leaves and stalks of Coix are nontoxic.