Objective: To prepare chitosan-Kudingcha nanoparticles and investigate its physicochemical properties and in vitro release characteristics. Method: Total flavonoids in Ilex kudingcha were extracted by gradient ethanol and its content was determined by UV-spectrophotometer

detection wavelength was 510 nm.Chitosan-Kudingcha nanoparticles were prepared by ionic crosslinking method

morphology

particle distribution

drug-loading

encapsulation efficiency and in vitro release rateof this preparation were examined. Result: The content of total flavonoids in I. kudingcha was 31.84%.These nanoparticles were spherical in shape and the size range was between 200-400 nm

the polydispersity coefficient was 0.137.Optimal prescription of chitosan-Kudingcha nanoparticles was as following:concentrations of chitosan

sodium tripolyphosphate and total flavonoids of 1 g · L-1.In vitro release profile showed that these nanoparticles had sudden release effect in the first two hours with cumulative release amount of 45.6% to 48.9%.Total release amount was 91.9% in 24 hours. Conclusion: Chitosan nanoparticles is an excellent carrier for I. kudingcha.This study can develop a new idea for development of lipid-lowering medicinal preparation.