Objective: To investigate effects of different permeation enhancer on in vitro transdermal permeation of glabridin through rat's skin and provide a reference for development of transdermal drug delivery system of this compound. Method: HPLC was adopted to determine the content of glabridin with mobile phase of acetonitrile-water-glacial acetic acid(55:44:1) and wavelength at 282 nm.The back skin of rats was used

Franz vertical diffusion cell was adopted to investigate transdermal performance of glabridin

effects of azone

borneol and propylene glycol on transdermal permeation of glabridin were observed. Result: Glabridin was difficult to penetrate the skin by itself

but it could penetrate the skin effectively by adding permeation enhancer.Effect of enhancers on glabridin was ranked as follows:5% azone > 5% propylene glycol > 5% borneol > monomer

permeation rates of steady state were 4.938

3.041

2.950 and 2.583 μg · cm-2 · h-1

the lag time were 0.056

0.180

0.059 and 0.167 h

respectively. Conclusion: Each penetration enhancers can promote transdermal effect of glabridin

and azone is the best one.