Objective: The extraction and purification methods for phenolics in Morus alba leaves were studied and the constituents of phenolic were identified

provided suitable experimental drug specimen for exploring the hypoglycemic effect of M. alba leaves. Method: The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. The factors affecting separation

such as resin dosage

pH and concentration of eluting agent were considerate. The chemical components of phenolic extracted from M. alba were analyzed and identified by UPLC-MS. Result: The optimized extracting conditions were as follows:extracted two times with 70% alcohol

each 1.5 h. The ratio of solvent to material 10:1 and 8:1

respectively;and the purification process with macroporous resin HPD-826 was using 1%Na2CO3

60% ethanol to wash out phenolics;eight phenolics compounds were identified by UPLC-MS. Conclusion: This method is simple

short cycle

low cost

high content and suitable for industrialized production. This purification technology could be used to extract phenolics of M. alba leaves for studies on its hypoglycemic effect.