咳特灵胶囊的UPLC指纹图谱及主成分的含量测定

员荣; 孙丽丽; 杨立伟; 管淑玉; 李将

doi:10.13422/j.cnki.syfjx.2015060037

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咳特灵胶囊的UPLC指纹图谱及主成分的含量测定

化学与分析 | 更新时间：2024-01-04

- 咳特灵胶囊的UPLC指纹图谱及主成分的含量测定
- Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC
- 中国实验方剂学杂志 2015年21卷第6期页码：37-40
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81102146)
- DOI：10.13422/j.cnki.syfjx.2015060037
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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员荣, 孙丽丽, 杨立伟, 等. 咳特灵胶囊的UPLC指纹图谱及主成分的含量测定[J]. 中国实验方剂学杂志, 2015,21(6):37-40.

YUAN Rong, SUN Li-li, YANG Li-wei, et al. Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 37-40.
员荣, 孙丽丽, 杨立伟, 等. 咳特灵胶囊的UPLC指纹图谱及主成分的含量测定[J]. 中国实验方剂学杂志, 2015,21(6):37-40. DOI： 10.13422/j.cnki.syfjx.2015060037.

YUAN Rong, SUN Li-li, YANG Li-wei, et al. Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 37-40. DOI： 10.13422/j.cnki.syfjx.2015060037.

摘要

目的: 建立咳特灵胶囊的超高效液相色谱指纹图谱方法

并测定牡荆苷和异牡荆苷的含量。方法: 采用Acquity UPLC HSS T3C18色谱柱(2.1 mm×100 mm

1.7 μm)

柱温25 ℃

流动相甲醇-0.05%甲酸水(梯度洗脱)

流速0.2 mL ·min-1

进样体积2 μL

检测波长325 nm。采用"中药色谱指纹图谱相似度评价系统"(2012.130723版)软件计算相似度;利用Acquity Waters system进行含量测定。结果: 在14批咳特灵胶囊测定的基础上

确立了对照指纹图谱

指出13个共有峰并对牡荆苷及异牡荆苷进行含量测定。结论: 所建立的UPLC指纹图谱方法及含量测定方法准确、重复性好

有较好稳定性

能够表征咳特灵胶囊的整体质量

可为其生产和质量控制提供科学的依据。

Abstract

Objective: To establish the fingerprint of the Keteling capsules and content determination of vitexin and isovitexin by UPLC. Method: The Acquity UPLC HSS C18(2.1 mm×100 mm

1.7 μm)column was used

the column temperature was 25 ℃

the mobile phase consisted of methanol-0.05% formic acid with gradient elution. The flow rate was 0.2 mL · min-1

and the detection wavelength was 325 nm. Fingerprint Similarity Evaluation Software(edition 2012.130723) was used to calculate the similarity and Acquity Waters system was used for the assay. Result: The fingerprint of the Keteling capsules was established

13 characteristic peaks were identified and the content of vitexin and isovitexin were determined. Conclusion: Established the UPLC fingerprint and content determination methods and reproducibility with good precision and stability

which could manifest the overall quality characteristics of the Keteling capsules. It could provide a scientific basis for the production and quality control of the Keteling capsules.

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